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Home > Product > ELISA Kit > Human Netrin Receptor Unc5B, UNC5B ELISA Kit

Human Netrin Receptor Unc5B, UNC5B ELISA Kit

Human Netrin Receptor Unc5B, UNC5B ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.052ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.052ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Netrin Receptor UNC5B

Code

E4182hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.052ng/ml

Detection range

0.1-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion (By similarity). Functions as netrin receptor that negatively regulates vascular branching during angiogenesis. Mediates retraction of tip cell filopodia on endothelial growth cones in response to netrin (By similarity). It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand (PubMed: 12598906). Mediates apoptosis by activating DAPK1. In the absence of NTN1, activates DAPK1 by reducing its autoinhibitory phosphorylation at Ser-308 thereby increasing its catalytic activity (By similarity).

UniProt accession

MASS(Da)

103,638

GeneID

219699

Synonyms

Netrin receptor UNC5B;p53RDL1;p53-regulated receptor for death and life protein 1;Protein unc-5 homolog 2;Protein unc-5 homolog B;UNC5B

Gene names

UNC5B

Research area

Neuroscience

Target protein

UNC5B

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4182hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 2.45 2.429 2.394
2.408
12ng/ml 1.57 1.549 1.514
1.529
6ng/ml 0.911 0.872 0.837
0.832
3ng/ml 0.404 0.444 0.409
0.484
1.5ng/ml 0.228 0.225 0.19
0.221
0ng/ml 0.039 0.035 0
0.03

Precision

We measured random samples of E4182hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3.3 0.16 4.9
Intra-Assay 2 18 14.6 0.62 4.2
Intra-Assay 3 18 8.7 0.49 5.6

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E4182hu

    $320.00

  • 96T

    E4182hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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