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Home > Product > ELISA Kit > Human Kappa-type Opioid Receptor, OPRK1 ELISA Kit

Human Kappa-type Opioid Receptor, OPRK1 ELISA Kit

Human Kappa-type Opioid Receptor, OPRK1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.019ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.019ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Kappa-Type Opioid Receptor

Code

E4216hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.019ng/ml

Detection range

0.05-15ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

G-protein coupled opioid receptor that functions as receptor for endogenous alpha-neoendorphins and dynorphins, but has low affinity for beta-endorphins. Also functions as receptor for various synthetic opioids and for the psychoactive diterpene salvinorin A. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain. Plays a role in mediating reduced physical activity upon treatment with synthetic opioids. Plays a role in the regulation of salivation in response to synthetic opioids. May play a role in arousal and regulation of autonomic and neuroendocrine functions.

UniProt accession

MASS(Da)

42,645

GeneID

4986

Synonyms

Kappa-type opioid receptor;KOR-1;K-OR-1;OPRK 1;OPRK1

Gene names

OPRK1

Research area

Others

Target protein

OPRK1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4216hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8ng/ml 2.266 2.192 2.152
2.119
4ng/ml 1.219 1.198 1.158
1.176
2ng/ml 0.748 0.723 0.683
0.699
1ng/ml 0.385 0.454 0.414
0.523
0.5ng/ml 0.171 0.203 0.163
0.235
0ng/ml 0.046 0.04 0
0.035

Precision

We measured random samples of E4216hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 4.9 0.16 3.3
Intra-Assay 2 18 0.9 0.05 6
Intra-Assay 3 18 2.3 0.13 5.6

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E4216hu

    $320.00

  • 96T

    E4216hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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