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Home > Product > ELISA Kit > Human Delta-aminolevulinic Acid Dehydratase, ALAD ELISA Kit

Human Delta-aminolevulinic Acid Dehydratase, ALAD ELISA Kit

Human Delta-aminolevulinic Acid Dehydratase, ALAD ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.027ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.027ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Delta-Aminolevulinic Acid Dehydratase

Code

E4217hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.027ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.

UniProt accession

MASS(Da)

36,295

GeneID

210

Synonyms

ALAD;ALADH;Delta-aminolevulinic acid dehydratase;Porphobilinogen Synthase

Gene names

ALAD

Research area

Cardiovascular

Target protein

ALAD

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4217hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.238 2.194 2.142
2.149
6ng/ml 1.842 1.799 1.747
1.755
3ng/ml 1.209 1.262 1.21
1.315
1.5ng/ml 0.738 0.726 0.674
0.714
0.75ng/ml 0.372 0.397 0.345
0.422
0ng/ml 0.041 0.052 0
0.062

Precision

We measured random samples of E4217hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 4.4 0.22 5.1
Intra-Assay 2 18 0.7 0.03 4.5
Intra-Assay 3 18 2.2 0.13 5.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E4217hu

    $320.00

  • 96T

    E4217hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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