Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

Background

UniProt accession

MASS（Da）

GeneID

Synonyms

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

These standard curves of E4221hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/L	2.188	2.281	2.215
	2.375
400ng/L	1.394	1.33	1.263
	1.265
200ng/L	0.686	0.702	0.635
	0.717
100ng/L	0.53	0.501	0.435
	0.473
50ng/L	0.271	0.29	0.224
	0.309
0ng/L	0.072	0.066	0
	0.061

We measured random samples of E4221hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	198	13.13	6.6
Intra-Assay	2	18	359.1	21.54	6
Intra-Assay	3	18	427.8	11.02	2.6

> Protocol Booklet

• Rat Flotillin-1, FLOT1 ELISA Kit