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Home > Product > ELISA Kit > Human Prostaglandin E Synthase, PTGES ELISA Kit

Human Prostaglandin E Synthase, PTGES ELISA Kit

Human Prostaglandin E Synthase, PTGES ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.57ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.57ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Prostaglandin E Synthase

Code

E4229hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.57ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Terminal enzyme of the cyclooxygenase (COX)-2-mediated prostaglandin E2 (PGE2) biosynthetic pathway. Catalyzes the glutathione-dependent oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2) in response to inflammatory stimuli (PubMed: 18682561, PubMed: 10377395, PubMed: 12672824, PubMed: 12460774, PubMed: 10869354, PubMed: 12244105). Plays a key role in inflammation response, fever and pain (By similarity). Catalyzes also the oxidoreduction of endocannabinoids into prostaglandin glycerol esters and PGG2 into 15-hydroperoxy-PGE2 (PubMed: 12244105, PubMed: 12672824). In addition, displays low glutathione transferase and glutathione-dependent peroxidase activities, toward 1-chloro-2,4-dinitrobenzene and 5-hydroperoxyicosatetraenoic acid (5-HPETE), respectively (PubMed: 12672824).

UniProt accession

MASS(Da)

17,102

GeneID

9536

Synonyms

Glutathione peroxidase PTGES;Glutathione transferase PTGES;MGST1-L1;Microsomal glutathione S-transferase 1-like 1;Microsomal prostaglandin E Synthase 1;MPGES-1;p53-induced gene 12 protein;Prostaglandin E Synthase;PTGES;

Gene names

PTGES

Research area

Signal Transduction

Target protein

PTGES

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4229hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.17 2.176 2.149
2.183
800ng/L 1.394 1.479 1.452
1.564
400ng/L 0.743 0.651 0.624
0.559
200ng/L 0.345 0.371 0.344
0.398
100ng/L 0.187 0.194 0.167
0.202
0ng/L 0.021 0.027 0
0.033

Precision

We measured random samples of E4229hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 736.2 19.69 2.7
Intra-Assay 2 18 505.9 21.08 4.2
Intra-Assay 3 18 357.2 13.99 3.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E4229hu

    $320.00

  • 96T

    E4229hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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