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Home > Product > ELISA Kit > Human Antithrombin 3, AT3 ELISA Kit

Human Antithrombin 3, AT3 ELISA Kit

Human Antithrombin 3, AT3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.96ng/mL

Detection range: 5-1000ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 2.96ng/mL

Detection range: 5-1000ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Antithrombin 3

Code

E4267hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.96ng/mL

Detection range

5-1000ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.

UniProt accession

MASS(Da)

52,602

GeneID

462

Synonyms

Antithrombin-III;ATIII;Serpin C1;SERPINC 1;SERPINC1

Gene names

SERPINC1

Research area

Cardiovascular

Target protein

SERPINC1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4267hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
640ng/mL 2.039 2.051 1.996
2.063
320ng/mL 1.655 1.69 1.634
1.724
160ng/mL 1.1 1.11 1.055
1.121
80ng/mL 0.695 0.694 0.639
0.694
40ng/mL 0.475 0.476 0.421
0.478
0ng/mL 0.055 0.055 0
0.055

Precision

We measured random samples of E4267hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 169 10.53 6.2
Intra-Assay 2 18 222.7 11.33 5.1
Intra-Assay 3 18 43.5 3.18 7.3

Protocols

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  • Size

    Code

    Price

  • 48T

    E4267hu

    $320.00

  • 96T

    E4267hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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