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Home > Product > ELISA Kit > Human Fumarate Hydratase, Mitochondrial, FH ELISA Kit

Human Fumarate Hydratase, Mitochondrial, FH ELISA Kit

Human Fumarate Hydratase, Mitochondrial, FH ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.88ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.88ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Fumarate Hydratase, Mitochondrial

Code

E4337hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.88ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes the reversible stereospecific interconversion of fumarate to L-malate (PubMed: 30761759). Experiments in other species have demonstrated that specific isoforms of this protein act in defined pathways and favor one direction over the other (Probable). [Isoform Mitochondrial]: Catalyzes the hydration of fumarate to L-malate in the tricarboxylic acid (TCA) cycle to facilitate a transition step in the production of energy in the form of NADH. [Isoform Cytoplasmic]: Catalyzes the dehydration of L-malate to fumarate (By similarity). Fumarate metabolism in the cytosol plays a role during urea cycle and arginine metabolism; fumarate being a by-product of the urea cycle and amino-acid catabolism (By similarity). Also plays a role in DNA repair by promoting non-homologous end-joining (NHEJ) (PubMed: 20231875, PubMed: 26237645). In response to DNA damage and phosphorylation by PRKDC, translocates to the nucleus and accumulates at DNA double-strand breaks (DSBs): acts by catalyzing formation of fumarate, an inhibitor of KDM2B histone demethylase activity, resulting in enhanced dimethylation of histone H3 'Lys-36' (H3K36me2) (PubMed: 26237645).

UniProt accession

MASS(Da)

54,637

GeneID

2271

Synonyms

FH;Fumarase;Fumarate hydratase, mitochondrial;HsFH

Gene names

FH

Research area

Others

Target protein

FH

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4337hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.018 1.944 1.903
1.87
1200ng/L 0.979 1.039 0.998
1.099
600ng/L 0.542 0.485 0.444
0.429
300ng/L 0.266 0.205 0.164
0.144
150ng/L 0.136 0.105 0.063
0.073
0ng/L 0.059 0.041 0
0.023

Precision

We measured random samples of E4337hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1449.1 37.26 2.6
Intra-Assay 2 18 1183.4 41.65 3.5
Intra-Assay 3 18 777.2 31.13 4

Protocols

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  • Size

    Code

    Price

  • 48T

    E4337hu

    $320.00

  • 96T

    E4337hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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