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Home > Product > ELISA Kit > Human Cytoplasmic Aconitate Hydratase, ACO1 ELISA Kit

Human Cytoplasmic Aconitate Hydratase, ACO1 ELISA Kit

Human Cytoplasmic Aconitate Hydratase, ACO1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.03ng/mL

Detection range: 2-32ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 1.03ng/mL

Detection range: 2-32ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Cytoplasmic Aconitate Hydratase

Code

E4338hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.03ng/mL

Detection range

2-32ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding. Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.

UniProt accession

MASS(Da)

98,399

GeneID

48

Synonyms

ACO 1;ACO1;Aconitase;Citrate hydro-lyase;Cytoplasmic aconitate hydratase;Ferritin repressor protein;IRE-BP 1;Iron regulatory protein 1;Iron-responsive element-binding protein 1;IRP1

Gene names

ACO1

Research area

Cardiovascular

Target protein

ACO1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4338hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
32ng/mL 2.356 2.332 2.302
2.308
16ng/mL 1.212 1.28 1.25
1.348
8ng/mL 0.559 0.621 0.591
0.682
4ng/mL 0.428 0.434 0.404
0.44
2ng/mL 0.216 0.188 0.158
0.16
0ng/mL 0.03 0.03 0
0.03

Precision

We measured random samples of E4338hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 10.2 0.44 4.3
Intra-Assay 2 18 15.8 0.36 2.3
Intra-Assay 3 18 6.2 0.38 6.2

Protocols

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  • Size

    Code

    Price

  • 48T

    E4338hu

    $320.00

  • 96T

    E4338hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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