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Home > Product > ELISA Kit > Canine Interleukin 21, IL-21 ELISA Kit

Canine Interleukin 21, IL-21 ELISA Kit

Canine Interleukin 21, IL-21 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 1.02ng/L

Detection range: 2-600ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Canine

Product Summary

Size: 96T,48T

Sensitivity: 1.02ng/L

Detection range: 2-600ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Canine

Product overview

Full product name

Interleukin 21

Code

E0075Ca

Assay type

Sandwich

Size

96T,48T

Sensitivity

1.02ng/L

Detection range

2-600ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Canine

Storage

2-8ºC

Assay time

1h 30m

Background

Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells (By similarity). During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation (By similarity).

UniProt accession

MASS(Da)

16,776

GeneID

442935

Synonyms

IL 21;IL21;IL-21;Interleukin-21

Gene names

IL21

Research area

Immunology; Cancer

Target protein

IL21

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0075Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
400ng/L 2.069 2.201 2.155
2.333
200ng/L 1.764 1.772 1.727
1.781
100ng/L 1.112 1.174 1.128
1.236
50ng/L 0.622 0.613 0.567
0.604
25ng/L 0.445 0.444 0.399
0.444
0ng/L 0.042 0.046 0
0.05

Precision

We measured random samples of E0075Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 44 2.23 5.1
Intra-Assay 2 18 160.9 6.9 4.3
Intra-Assay 3 18 100.9 6.59 6.5

Protocols

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  • Size

    Code

    Price

  • 96T

    E0075Ca

    $475.00

  • 48T

    E0075Ca

    $350.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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