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Home > Product > ELISA Kit > Human C-C Motif Chemokine 25, CCL25 ELISA Kit

Human C-C Motif Chemokine 25, CCL25 ELISA Kit

Human C-C Motif Chemokine 25, CCL25 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 3.43ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 3.43ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

C-C Motif Chemokine 25

Code

E4392hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

3.43ng/L

Detection range

7-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.

UniProt accession

MASS(Da)

16,609

GeneID

6370

Synonyms

C-C motif chemokine 25;CCL 25;CCL25;Chemokine TECK;Small-inducible cytokine A25;Thymus-expressed chemokine

Gene names

CCL25

Research area

Immunology

Target protein

CCL25

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4392hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/L 2.38 2.358 2.322
2.335
400ng/L 1.197 1.254 1.218
1.31
200ng/L 0.827 0.811 0.775
0.795
100ng/L 0.462 0.445 0.41
0.429
50ng/L 0.205 0.23 0.194
0.254
0ng/L 0.039 0.036 0
0.032

Precision

We measured random samples of E4392hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 480.4 15.21 3.2
Intra-Assay 2 18 113.7 7.27 6.4
Intra-Assay 3 18 295.3 19.27 6.5

Protocols

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  • Size

    Code

    Price

  • 48T

    E4392hu

    $320.00

  • 96T

    E4392hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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