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Home > Product > ELISA Kit > Human Moesin, MSN ELISA Kit

Human Moesin, MSN ELISA Kit

Human Moesin, MSN ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.47ng/L

Detection range: 200-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.47ng/L

Detection range: 200-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Moesin

Code

E4441Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.47ng/L

Detection range

200-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Ezrin-radixin-moesin (ERM) family protein that connects the actin cytoskeleton to the plasma membrane and thereby regulates the structure and function of specific domains of the cell cortex. Tethers actin filaments by oscillating between a resting and an activated state providing transient interactions between moesin and the actin cytoskeleton (PubMed: 10212266). Once phosphorylated on its C-terminal threonine, moesin is activated leading to interaction with F-actin and cytoskeletal rearrangement (PubMed: 10212266). These rearrangements regulate many cellular processes, including cell shape determination, membrane transport, and signal transduction (PubMed: 12387735, PubMed: 15039356). The role of moesin is particularly important in immunity acting on both T and B-cells homeostasis and self-tolerance, regulating lymphocyte egress from lymphoid organs (PubMed: 9298994, PubMed: 9616160). Modulates phagolysosomal biogenesis in macrophages (By similarity). Participates also in immunologic synapse formation (PubMed: 27405666).

UniProt accession

MASS(Da)

67,820

GeneID

4478

Synonyms

Membrane-organizing extension spike protein;Moesin;MSN

Gene names

MSN

Research area

Signal Transduction

Target protein

MSN

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4441Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.184 2.223 2.172
2.263
1200ng/L 1.442 1.433 1.381
1.423
600ng/L 0.827 0.823 0.772
0.819
300ng/L 0.518 0.463 0.412
0.409
150ng/L 0.272 0.291 0.24
0.31
0ng/L 0.053 0.051 0
0.049

Precision

We measured random samples of E4441Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1167 46.04 3.9
Intra-Assay 2 18 237.7 13.68 5.8
Intra-Assay 3 18 945.3 51.88 5.5

Protocols

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  • Size

    Code

    Price

  • 48T

    E4441Hu

    $320.00

  • 96T

    E4441Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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