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Home > Product > ELISA Kit > Human Interleukin 36 Beta, IL-36B ELISA Kit

Human Interleukin 36 Beta, IL-36B ELISA Kit

Human Interleukin 36 Beta, IL-36B ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.24ng/L

Detection range: 0.5-150ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.24ng/L

Detection range: 0.5-150ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Interleukin 36 Beta

Code

E4463Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.24ng/L

Detection range

0.5-150ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Stimulates production of interleukin-6 and interleukin-8 in synovial fibrobasts, articular chondrocytes and mature adipocytes. Induces expression of a number of antimicrobial Peptides including beta-defensins 4 and 103 as well as a number of matrix metalloproteases. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1, and the production of proinflammatory cytokines such as TNF-alpha, IL-8 and IL-6.

UniProt accession

MASS(Da)

18,522

GeneID

27177

Synonyms

FIL1 eta;IL 36B;IL-1 eta;IL-1F8;IL-1H2;IL36B;Interleukin-1 eta;Interleukin-1 family member 8;Interleukin-1 homolog 2;Interleukin-36 beta

Gene names

IL36B

Research area

Immunology

Target protein

IL36B

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4463Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
80ng/L 2.083 2.02 1.97
1.956
40ng/L 1.386 1.368 1.319
1.349
20ng/L 0.541 0.629 0.58
0.717
10ng/L 0.436 0.419 0.37
0.403
5ng/L 0.211 0.166 0.116
0.12
0ng/L 0.057 0.049 0
0.041

Precision

We measured random samples of E4463Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 26.7 1.51 5.7
Intra-Assay 2 18 44.4 1.52 3.4
Intra-Assay 3 18 20.6 1.11 5.4

Protocols

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  • Size

    Code

    Price

  • 48T

    E4463Hu

    $320.00

  • 96T

    E4463Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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