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Home > Product > ELISA Kit > Human Receptor-interacting Serine-Threonine Kinase 3, PIPK3 ELISA Kit

Human Receptor-interacting Serine-Threonine Kinase 3, PIPK3 ELISA Kit

Human Receptor-interacting Serine-Threonine Kinase 3, PIPK3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 4.59ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 4.59ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Receptor-Interacting Serine-Threonine Kinase 3

Code

E4492Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

4.59ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Serine/threonine-protein kinase that activates necroptosis and apoptosis, two parallel forms of cell death (PubMed: 19524512, PubMed: 19524513, PubMed: 22265413, PubMed: 22265414, PubMed: 22421439, PubMed: 29883609). Necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members, is triggered by RIPK3 following activation by ZBP1 (PubMed: 19524512, PubMed: 19524513, PubMed: 22265413, PubMed: 22265414, PubMed: 22421439, PubMed: 29883609, PubMed: 32298652). Activated RIPK3 forms a necrosis-inducing complex and mediates phosphorylation of MLKL, promoting MLKL localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage (PubMed: 19524512, PubMed: 19524513, PubMed: 22265413, PubMed: 22265414, PubMed: 22421439, PubMed: 25316792, PubMed: 29883609). In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol (By similarity). Also regulates apoptosis: apoptosis depends on RIPK1, FADD and CASP8, and is independent of MLKL and RIPK3 kinase activity (By similarity). Phosphorylates RIPK1: RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation (PubMed: 19524513). In some cell types, also able to restrict viral replication by promoting cell death-independent responses (By similarity). In response to Zika virus infection in neurons, promotes a cell death-independent pathway that restricts viral replication: together with ZBP1, promotes a death-independent transcriptional program that modifies the cellular metabolism via up-regulation expression of the enzyme ACOD1/IRG1 and production of the metabolite itaconate (By similarity). Itaconate inhibits the activity of succinate dehydrogenase, generating a metabolic state in neurons that suppresses replication of viral genomes (By similarity). RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL (PubMed: 19$498.00|$360.00109). These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production (PubMed: 19$498.00|$360.00109).

UniProt accession

MASS(Da)

56,887

GeneID

11035

Synonyms

Receptor-interacting protein 3;Receptor-interacting serine/threonine-protein kinase 3;RIP-3;RIPK 3;RIPK3;RIP-like protein kinase 3

Gene names

RIPK3

Research area

Immunology

Target protein

RIPK3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4492Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.255 2.132 2.079
2.009
600ng/L 1.283 1.323 1.27
1.363
300ng/L 0.851 0.872 0.819
0.892
150ng/L 0.442 0.45 0.397
0.458
75ng/L 0.262 0.242 0.19
0.223
0ng/L 0.055 0.053 0
0.051

Precision

We measured random samples of E4492Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 424.2 26.11 6.2
Intra-Assay 2 18 757.5 29.64 3.9
Intra-Assay 3 18 255.7 18.24 7.1

Protocols

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  • Size

    Code

    Price

  • 48T

    E4492Hu

    $320.00

  • 96T

    E4492Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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