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Home > Product > ELISA Kit > Human Cytochrome Coxidase Subunit 2, TNNI1 ELISA Kit

Human Cytochrome Coxidase Subunit 2, TNNI1 ELISA Kit

Human Cytochrome Coxidase Subunit 2, TNNI1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 12.29ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 12.29ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Cytochrome C Oxidase Subunit 2

Code

E4613Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

12.29ng/L

Detection range

20-6000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP Synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

UniProt accession

MASS(Da)

25,565

GeneID

4513

Synonyms

Cytochrome c oxidase polypeptide II;Cytochrome c oxidase subunit 2;MT CO2;MT-CO2

Gene names

MT-CO2

Research area

Others

Target protein

MT-CO2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4613Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
3200ng/L 2.057 2.156 2.122
2.255
1600ng/L 1.379 1.421 1.386
1.462
800ng/L 0.558 0.578 0.543
0.598
400ng/L 0.328 0.373 0.338
0.418
200ng/L 0.139 0.159 0.124
0.178
0ng/L 0.038 0.035 0
0.032

Precision

We measured random samples of E4613Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1189.8 43.21 3.6
Intra-Assay 2 18 1495 28.8 1.9
Intra-Assay 3 18 846 36.79 4.3

Protocols

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  • Size

    Code

    Price

  • 48T

    E4613Hu

    $320.00

  • 96T

    E4613Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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