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Home > Product > ELISA Kit > Human Actin-related Protein 3, ACTR3 ELISA Kit

Human Actin-related Protein 3, ACTR3 ELISA Kit

Human Actin-related Protein 3, ACTR3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 12.05ng/L

Detection range: 20-4000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 12.05ng/L

Detection range: 20-4000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Actin-Related Protein 3

Code

E4623Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

12.05ng/L

Detection range

20-4000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed: 9000076). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed: 9000076). Seems to contact the pointed end of the daughter actin filament (PubMed: 9000076). In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation (PubMed: 29058690). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed: 17220302, PubMed: 29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed: 29925947). Plays a role in ciliogenesis (PubMed: 20393563).

UniProt accession

MASS(Da)

47,371

GeneID

10096

Synonyms

Actin-like protein 3;Actin-related protein 3;ACTR 3;ACTR3

Gene names

ACTR3

Research area

Others

Target protein

ACTR3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4623Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.088 2.237 2.189
2.387
1200ng/L 1.342 1.369 1.321
1.397
600ng/L 0.579 0.658 0.61
0.736
300ng/L 0.439 0.41 0.362
0.38
150ng/L 0.19 0.193 0.145
0.195
0ng/L 0.054 0.048 0
0.042

Precision

We measured random samples of E4623Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1404.1 41.8 3
Intra-Assay 2 18 1061.2 44.8 4.2
Intra-Assay 3 18 483.9 22.57 4.7

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E4623Hu

    $320.00

  • 96T

    E4623Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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