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Home > Product > ELISA Kit > Human Cofilin-1, CFL1 ELISA Kit

Human Cofilin-1, CFL1 ELISA Kit

Human Cofilin-1, CFL1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.96ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.96ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Cofilin-1

Code

E4625Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.96ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed: 11812157). In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed: 15580268). Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells (PubMed: 2183$498.00|$360.007). Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed: 23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity).

UniProt accession

MASS(Da)

18,502

GeneID

1072

Synonyms

18 kDa phosphoprotein;CFL 1;CFL1;Cofilin, non-muscle isoform;Cofilin-1;p18

Gene names

CFL1

Research area

Signal Transduction

Target protein

CFL1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4625Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.378 2.489 2.455
2.6
800ng/L 1.558 1.524 1.49
1.49
400ng/L 0.815 0.795 0.76
0.774
200ng/L 0.483 0.486 0.452
0.49
100ng/L 0.287 0.286 0.252
0.285
0ng/L 0.035 0.034 0
0.033

Precision

We measured random samples of E4625Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 695.5 23.18 3.3
Intra-Assay 2 18 289.4 20.23 7
Intra-Assay 3 18 599.3 32.85 5.5

Protocols

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  • Size

    Code

    Price

  • 48T

    E4625Hu

    $320.00

  • 96T

    E4625Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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