Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids . APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance . Apoliproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma . As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL) .
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard solution | 0.5ml x1 |
Standard diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop solution | 6ml x1 |
Substrate solution A | 6ml x1 |
Substrate solution B | 6ml x1 |
Wash buffer concentrate (25x) | 20ml x1 |
Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E4761Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
32μg/ml | 2.399 | 2.508 | 2.461 |
2.617 | |||
16μg/ml | 1.429 | 1.439 | 1.392 |
1.449 | |||
8μg/ml | 0.932 | 0.896 | 0.85 |
0.861 | |||
4μg/ml | 0.398 | 0.403 | 0.356 |
0.407 | |||
2μg/ml | 0.202 | 0.24 | 0.194 |
0.278 | |||
0μg/ml | 0.059 | 0.047 | 0 |
0.035 |
Precision
We measured random samples of E4761Hu within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-Assay | 1 | 18 | 11.5 | 0.48 | 4.2 |
Intra-Assay | 2 | 18 | 17.3 | 0.45 | 2.6 |
Intra-Assay | 3 | 18 | 3.2 | 0.2 | 6.3 |
Protocols
References
Customer reviews and Q&As