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Home > Product > ELISA Kit > Human C-C Motif Chemokine 4, CCL4 ELISA Kit

Human C-C Motif Chemokine 4, CCL4 ELISA Kit

Human C-C Motif Chemokine 4, CCL4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.12ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 5.12ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

C-C Motif Chemokine 4

Code

E5008Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.12ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.

UniProt accession

MASS(Da)

10,212

GeneID

3883726351

Synonyms

ACT-2;C-C motif chemokine 4;CCL 4;CCL4;G-26 T-lymphocyte-secreted protein;HC21;LAG-1;Lymphocyte activation gene 1 protein;Macrophage inflammatory protein 1-beta;MIP-1-beta;MIP-1-beta(1-69;MIP-1-beta(3-69;PAT 744;Protein H400;SIS-gamma;Small-inducible cytokine A4;T-cell activation protein 2

Gene names

CCL4

Research area

Immunology

Target protein

CCL4

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5008Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.134 2.245 2.195
2.357
600ng/L 1.335 1.36 1.31
1.385
300ng/L 0.823 0.77 0.72
0.718
150ng/L 0.473 0.479 0.429
0.486
75ng/L 0.188 0.198 0.148
0.209
0ng/L 0.041 0.05 0
0.059

Precision

We measured random samples of E5008Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 388.3 17.95 4.6
Intra-Assay 2 18 809.1 22.89 2.8
Intra-Assay 3 18 182.4 10.68 5.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E5008Hu

    $320.00

  • 96T

    E5008Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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