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Home > Product > ELISA Kit > Human Centromere Protein K, CENPK ELISA Kit

Human Centromere Protein K, CENPK ELISA Kit

Human Centromere Protein K, CENPK ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 6.95ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 6.95ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Centromere Protein K

Code

E5030Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

6.95ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with KNL1 to recruit the NDC80 complex to the outer kinetochore.

UniProt accession

MASS(Da)

31,655

GeneID

64105

Synonyms

CENPK;CENP-K;Centromere protein K;Interphase centromere complex protein 37;p33;Protein AF-5alpha

Gene names

CENPK

Research area

Others

Target protein

CENPK

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5030Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.111 2.081 2.029
2.051
800ng/L 1.388 1.366 1.313
1.343
400ng/L 0.861 0.91 0.858
0.96
200ng/L 0.443 0.433 0.381
0.423
100ng/L 0.294 0.282 0.23
0.27
0ng/L 0.045 0.052 0
0.059

Precision

We measured random samples of E5030Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 204.5 11.16 5.5
Intra-Assay 2 18 1185.7 55.87 4.7
Intra-Assay 3 18 509.1 31.39 6.2

Protocols

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  • Size

    Code

    Price

  • 48T

    E5030Hu

    $320.00

  • 96T

    E5030Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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