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Home > Product > ELISA Kit > Human Centromere Protein M, CENPM ELISA Kit

Human Centromere Protein M, CENPM ELISA Kit

Human Centromere Protein M, CENPM ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.17ng/mL

Detection range: 0.3-90ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.17ng/mL

Detection range: 0.3-90ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Centromere Protein M

Code

E5031Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.17ng/mL

Detection range

0.3-90ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres.

UniProt accession

MASS(Da)

19,737

GeneID

79019

Synonyms

CENPM;CENP-M;Centromere protein M;Interphase centromere complex protein 39;Proliferation-associated nuclear element protein 1

Gene names

CENPM

Research area

Epigenetics and Nuclear Signaling

Target protein

CENPM

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5031Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
48ng/mL 2.048 2.063 2.028
2.078
24ng/mL 0.984 1.007 0.971
1.03
12ng/mL 0.385 0.444 0.409
0.503
6ng/mL 0.317 0.298 0.262
0.278
3ng/mL 0.122 0.139 0.103
0.155
0ng/mL 0.036 0.036 0
0.035

Precision

We measured random samples of E5031Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 24.2 1.18 4.9
Intra-Assay 2 18 36.3 0.85 2.3
Intra-Assay 3 18 9.7 0.48 4.9

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E5031Hu

    $320.00

  • 96T

    E5031Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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