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Home > Product > ELISA Kit > Human Chondroitin Sulfate Proteoglycan 4, CSPG4 ELISA Kit

Human Chondroitin Sulfate Proteoglycan 4, CSPG4 ELISA Kit

Human Chondroitin Sulfate Proteoglycan 4, CSPG4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 6.91ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 6.91ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Chondroitin Sulfate Proteoglycan 4

Code

E5043Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

6.91ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.

UniProt accession

MASS(Da)

250,537

GeneID

1464

Synonyms

Chondroitin sulfate proteoglycan 4;Chondroitin sulfate proteoglycan NG2;CSPG 4;CSPG4;Melanoma chondroitin sulfate proteoglycan;Melanoma-associated chondroitin sulfate proteoglycan

Gene names

CSPG4

Research area

Cardiovascular

Target protein

CSPG4

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5043Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 1.956 1.935 1.885
1.914
800ng/L 0.88 0.894 0.845
0.909
400ng/L 0.532 0.586 0.537
0.64
200ng/L 0.284 0.282 0.233
0.28
100ng/L 0.083 0.111 0.062
0.14
0ng/L 0.062 0.049 0
0.037

Precision

We measured random samples of E5043Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1165.9 24.52 2.1
Intra-Assay 2 18 962.9 42.91 4.5
Intra-Assay 3 18 457.3 19.64 4.3

Protocols

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  • Size

    Code

    Price

  • 48T

    E5043Hu

    $320.00

  • 96T

    E5043Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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