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Home > Product > ELISA Kit > Canine C-C Motif Chemokine 5, CCL5 ELISA Kit

Canine C-C Motif Chemokine 5, CCL5 ELISA Kit

Canine C-C Motif Chemokine 5, CCL5 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.021ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Canine

Product Summary

Size: 96T,48T

Sensitivity: 0.021ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Canine

Product overview

Full product name

C-C Motif Chemokine 5

Code

E0337Ca

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.021ng/mL

Detection range

0.05-20ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Canine

Storage

2-8ºC

Assay time

1h 30m

Background

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.

UniProt accession

MASS(Da)

10,179

GeneID

403522

Synonyms

C-C motif chemokine 5;CCL 5;CCL5;Small-inducible cytokine A5;T-cell-specific protein RANTES

Gene names

CCL5

Research area

Immunology; Cardiovascular

Target protein

CCL5

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0337Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/mL 2.242 2.166 2.13
2.091
6ng/mL 1.694 1.637 1.6
1.579
3ng/mL 1.1 1.125 1.088
1.15
1.5ng/mL 0.685 0.712 0.675
0.738
0.75ng/mL 0.456 0.472 0.435
0.487
0ng/mL 0.044 0.037 0
0.03

Precision

We measured random samples of E0337Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 4.2 0.22 5.2
Intra-Assay 2 18 0.7 0.05 7.4
Intra-Assay 3 18 1.6 0.11 7

Protocols

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  • Size

    Code

    Price

  • 96T

    E0337Ca

    $475.00

  • 48T

    E0337Ca

    $350.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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