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Home > Product > ELISA Kit > Human Coiled-Coil Domain-containing Protein 134, CCDC134 ELISA Kit

Human Coiled-Coil Domain-containing Protein 134, CCDC134 ELISA Kit

Human Coiled-Coil Domain-containing Protein 134, CCDC134 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.021ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.021ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Coiled-Coil Domain-Containing Protein 134

Code

E5064Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.021ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

In extracellular secreted form, promotes proliferation and activation of CD8(+) T cells, suggesting a cytokine-like function (PubMed: 25125657). Enhances cytotoxic anti-tumor activity of CD8(+) T cells (PubMed: 25125657). May inhibit ERK and JNK signaling activity (PubMed: 18087676, PubMed: 23070808). May suppress cell migration and invasion activity, via its effects on ERK and JNK signaling (PubMed: 23070808). In the nucleus, enhances stability of the PCAF histone acetyltransferase (HAT) complex member TADA2A and thus promotes PCAF-mediated H3K14 and H4K8 HAT activity. May inhibit TADA2A-mediated TP53/p53 'Lys-321' acetylation, leading to reduced TP53 stability and transcriptional activity. May also promote TADA2A-mediated XRCC6 acetylation thus facilitating cell apoptosis in response to DNA damage.

UniProt accession

MASS(Da)

26,561

GeneID

79879

Synonyms

CCDC 134;CCDC134;Coiled-coil domain-containing protein 134

Gene names

CCDC134

Research area

Others

Target protein

CCDC134

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5064Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.199 2.176 2.135
2.153
6ng/ml 1.19 1.31 1.269
1.431
3ng/ml 0.772 0.742 0.701
0.712
1.5ng/ml 0.415 0.404 0.363
0.393
0.75ng/ml 0.215 0.204 0.163
0.194
0ng/ml 0.042 0.041 0
0.041

Precision

We measured random samples of E5064Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 7.7 0.27 3.5
Intra-Assay 2 18 3 0.18 6
Intra-Assay 3 18 4 0.2 4.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E5064Hu

    $320.00

  • 96T

    E5064Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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