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Home > Product > ELISA Kit > Human Cold-inducible RNA-binding Protein, CIRBP ELISA Kit

Human Cold-inducible RNA-binding Protein, CIRBP ELISA Kit

Human Cold-inducible RNA-binding Protein, CIRBP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 4.02ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 4.02ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Cold-Inducible Rna-Binding Protein

Code

E5066Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

4.02ng/L

Detection range

7-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor (By similarity). Promotes assembly of stress granules (SGs), when overexpressed.

UniProt accession

MASS(Da)

18,648

GeneID

1153

Synonyms

A18 hnRNP;CIRBP;Cold-inducible RNA-binding protein;Glycine-rich RNA-binding protein CIRP

Gene names

CIRBP

Research area

Neuroscience

Target protein

CIRBP

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5066Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/L 2.535 2.536 2.489
2.538
400ng/L 1.385 1.424 1.376
1.462
200ng/L 0.801 0.782 0.734
0.762
100ng/L 0.518 0.466 0.419
0.415
50ng/L 0.266 0.239 0.192
0.212
0ng/L 0.034 0.047 0
0.061

Precision

We measured random samples of E5066Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 112.6 6.16 5.5
Intra-Assay 2 18 525.3 16.06 3.1
Intra-Assay 3 18 329.3 17.37 5.3

Protocols

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  • Size

    Code

    Price

  • 48T

    E5066Hu

    $320.00

  • 96T

    E5066Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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