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Home > Product > ELISA Kit > Human Major Prion Protein, PRNP ELISA Kit

Human Major Prion Protein, PRNP ELISA Kit

Human Major Prion Protein, PRNP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.055ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.055ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Major Prion Protein

Code

E5109Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.055ng/ml

Detection range

0.1-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Its primary physiological function is unclear. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May promote myelin homeostasis through acting as an agonist for ADGRG6 receptor. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro) (By similarity). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or ZN(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains (By similarity).

UniProt accession

MASS(Da)

27,661

GeneID

5621

Synonyms

ASCR;CD antigen CD230;Major prion protein;PRNP;PrP;PrP27-30;PrP33-35C

Gene names

PRNP

Research area

Neuroscience

Target protein

PRNP

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5109Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 2.295 2.342 2.287
2.388
12ng/ml 1.305 1.347 1.292
1.389
6ng/ml 0.748 0.77 0.715
0.792
3ng/ml 0.456 0.506 0.451
0.556
1.5ng/ml 0.291 0.28 0.225
0.269
0ng/ml 0.06 0.055 0
0.051

Precision

We measured random samples of E5109Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 10.9 0.38 3.5
Intra-Assay 2 18 3.9 0.22 5.7
Intra-Assay 3 18 9.7 0.5 5.1

Protocols

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  • Size

    Code

    Price

  • 48T

    E5109Hu

    $320.00

  • 96T

    E5109Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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