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Home > Product > ELISA Kit > Human Cytochrome P450 2C9, CYP2C9 ELISA Kit

Human Cytochrome P450 2C9, CYP2C9 ELISA Kit

Human Cytochrome P450 2C9, CYP2C9 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.59ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.59ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Cytochrome P450 2C9

Code

E5160Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.59ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids and steroids (PubMed: 7574697, PubMed: 9866708, PubMed: 9435160, PubMed: 12865317, PubMed: 15766564, PubMed: 19965576, PubMed: 21576599). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed: 7574697, PubMed: 9866708, PubMed: 9435160, PubMed: 12865317, PubMed: 15766564, PubMed: 19965576, PubMed: 21576599). Catalyzes the epoxidation of double bonds of polyunsaturated fatty acids (PUFA) (PubMed: 7574697, PubMed: 15766564, PubMed: 19965576, PubMed: 9866708). Catalyzes the hydroxylation of carbon-hydrogen bonds. Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis (PubMed: 21576599). Exhibits low catalytic activity for the formation of catechol estrogens from 17beta-estradiol (E2) and estrone (E1), namely 2-hydroxy E1 and E2 (PubMed: 12865317). Catalyzes bisallylic hydroxylation and hydroxylation with double-bond migration of polyunsaturated fatty acids (PUFA) (PubMed: 9866708, PubMed: 9435160). Also metabolizes plant monoterpenes such as limonene. Oxygenates (R)- and (S)-limonene to produce carveol and perillyl alcohol (PubMed: 11950794). Contributes to the wide pharmacokinetics variability of the metabolism of drugs such as S-warfarin, diclofenac, phenytoin, tolbutamide and losartan (PubMed: 25994031).

UniProt accession

MASS(Da)

55,628

GeneID

1559

Synonyms

(R-limonene 6-monooxygenase;(S-limonene 6-monooxygenase;(S-limonene 7-monooxygenase;Cholesterol 25-hydroxylase;CYP2C9;CYPIIC9;Cytochrome P450 2C9;Cytochrome P450 MP-4;Cytochrome P450 MP-8;Cytochrome P450 PB-1;Cytochrome P-450MP;S-mephenytoin 4-hydroxylase

Gene names

CYP2C9

Research area

Cardiovascular

Target protein

CYP2C9

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5160Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.467 2.546 2.512
2.625
800ng/L 1.513 1.452 1.419
1.391
400ng/L 0.787 0.831 0.798
0.876
200ng/L 0.391 0.431 0.398
0.471
100ng/L 0.219 0.25 0.216
0.281
0ng/L 0.036 0.033 0
0.03

Precision

We measured random samples of E5160Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 761.5 26.75 3.5
Intra-Assay 2 18 447.1 22.23 5
Intra-Assay 3 18 306.3 19.19 6.3

Protocols

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  • Size

    Code

    Price

  • 48T

    E5160Hu

    $320.00

  • 96T

    E5160Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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