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Home > Product > ELISA Kit > Human Dedicator of Cytokinesis Protein 1, DOCK1 ELISA Kit

Human Dedicator of Cytokinesis Protein 1, DOCK1 ELISA Kit

Human Dedicator of Cytokinesis Protein 1, DOCK1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.72ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.72ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Dedicator of Cytokinesis Protein 1

Code

E5173Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.72ng/L

Detection range

20-6000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Along with DOCK1, mediates CRK/CRKL regulation of epithelial and endothelial cell spreading and migration on type IV collagen (PubMed: 19004829). Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1 (PubMed: 8657152).

UniProt accession

MASS(Da)

215,346

GeneID

1793

Synonyms

180 kDa protein downstream of CRK;Dedicator of cytokinesis protein 1;DOCK 1;DOCK1;DOCK180

Gene names

DOCK1

Research area

Others

Target protein

DOCK1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5173Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
3200ng/L 2.157 2.139 2.098
2.122
1600ng/L 1.55 1.586 1.544
1.622
800ng/L 0.966 0.966 0.925
0.967
400ng/L 0.779 0.735 0.694
0.692
200ng/L 0.428 0.445 0.404
0.462
0ng/L 0.031 0.041 0
0.052

Precision

We measured random samples of E5173Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 254.4 18.51 7.3
Intra-Assay 2 18 560.6 35.49 6.3
Intra-Assay 3 18 1095 54.14 4.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E5173Hu

    $320.00

  • 96T

    E5173Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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