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Home > Product > ELISA Kit > Human Dipeptidase 1, DPEP1 ELISA Kit

Human Dipeptidase 1, DPEP1 ELISA Kit

Human Dipeptidase 1, DPEP1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.63ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.63ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Dipeptidase 1

Code

E5186Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.63ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Hydrolyzes a wide range of dipeptides including the conversion of leukotriene D4 to leukotriene E4 (PubMed: 2303490, PubMed: 6334084, PubMed: 31442408). Hydrolyzes cystinyl-bis-glycine (cys-bis-gly) formed during glutathione degradation (By similarity). Possesses also beta lactamase activity and hydrolytically inactivates beta-lactam antibiotics (PubMed: 6334084). Independently of its dipeptidase activity acts as an adhesion receptor for neutrophils recruitement from bloodstream into inflammed lungs and liver.

UniProt accession

MASS(Da)

45,674

GeneID

1800

Synonyms

Beta-lactamase;Dehydropeptidase-I;Dipeptidase 1;DPEP 1;DPEP1;hRDP;Microsomal dipeptidase;Renal dipeptidase

Gene names

DPEP1

Research area

Cancer

Target protein

DPEP1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5186Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 1.989 1.845 1.806
1.701
800ng/L 0.915 0.899 0.86
0.883
400ng/L 0.434 0.536 0.497
0.637
200ng/L 0.219 0.245 0.206
0.271
100ng/L 0.094 0.094 0.055
0.094
0ng/L 0.026 0.039 0
0.051

Precision

We measured random samples of E5186Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 948 44.54 4.7
Intra-Assay 2 18 1419.9 38.79 2.7
Intra-Assay 3 18 544 27.03 5

Protocols

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  • Size

    Code

    Price

  • 48T

    E5186Hu

    $320.00

  • 96T

    E5186Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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