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Home > Product > ELISA Kit > Human DNA Replication Factor Cdt1, CDT1 ELISA Kit

Human DNA Replication Factor Cdt1, CDT1 ELISA Kit

Human DNA Replication Factor Cdt1, CDT1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.63ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.63ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Dna Replication Factor Cdt1

Code

E5191Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.63ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Required for both DNA replication and mitosis (PubMed: 11125146, PubMed: 22581055, PubMed: 21856198, PubMed: 14993212, PubMed: 26842564). DNA replication licensing factor, required for pre-replication complex assembly. Cooperates with CDC6 and the origin recognition complex (ORC) during G1 phase of the cell cycle to promote the loading of the mini-chromosome maintenance (MCM) complex onto DNA to generate pre-replication complexes (pre-RC)(PubMed: 14672932). Required also for mitosis by promoting stable kinetochore-microtubule attachments (PubMed: 22581055). Potential oncogene (By similarity).

UniProt accession

MASS(Da)

60,390

GeneID

81620

Synonyms

CDT 1;CDT1;DNA replication factor Cdt1;Double parked homolog;DUP

Gene names

CDT1

Research area

Epigenetics and Nuclear Signaling

Target protein

CDT1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5191Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.686 2.566 2.517
2.446
800ng/L 1.581 1.492 1.443
1.403
400ng/L 0.92 0.925 0.876
0.93
200ng/L 0.413 0.46 0.411
0.506
100ng/L 0.249 0.218 0.169
0.188
0ng/L 0.041 0.049 0
0.057

Precision

We measured random samples of E5191Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 668.2 22.18 3.3
Intra-Assay 2 18 464.4 23.9 5.1
Intra-Assay 3 18 181.4 10.7 5.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E5191Hu

    $320.00

  • 96T

    E5191Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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