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Home > Product > ELISA Kit > Human DNA-binding Protein Ikaros, IKZF1 ELISA Kit

Human DNA-binding Protein Ikaros, IKZF1 ELISA Kit

Human DNA-binding Protein Ikaros, IKZF1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 10.06ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 10.06ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

DNA-Binding Protein Ikaros

Code

E5197Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

10.06ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Transcription regulator of hematopoietic cell differentiation (PubMed: 17934067). Binds gamma-satellite DNA (PubMed: 17135265, PubMed: 19141594). Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity). Function is isoform-specific and is modulated by dominant-negative inactive isoforms (PubMed: 17135265, PubMed: 17934067).

UniProt accession

MASS(Da)

57,528

GeneID

10320

Synonyms

DNA-binding protein Ikaros;Ikaros family zinc finger protein 1;IKZF 1;IKZF1;Lymphoid transcription factor LyF-1

Gene names

IKZF1

Research area

Others

Target protein

IKZF1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5197Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.221 2.209 2.161
2.196
1200ng/L 1.207 1.257 1.209
1.306
600ng/L 0.854 0.798 0.751
0.742
300ng/L 0.52 0.498 0.45
0.475
150ng/L 0.23 0.206 0.158
0.181
0ng/L 0.043 0.047 0
0.052

Precision

We measured random samples of E5197Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1556 64.9 4.2
Intra-Assay 2 18 1090.8 70.89 6.5
Intra-Assay 3 18 516.8 35.95 7

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E5197Hu

    $320.00

  • 96T

    E5197Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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