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Home > Product > ELISA Kit > Human DNA-binding Protein Inhibitor Id-1, ID1 ELISA Kit

Human DNA-binding Protein Inhibitor Id-1, ID1 ELISA Kit

Human DNA-binding Protein Inhibitor Id-1, ID1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 10.11ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 10.11ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

DNA-Binding Protein Inhibitor ID-1

Code

E5198Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

10.11ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer (By similarity).

UniProt accession

MASS(Da)

16,133

GeneID

3397

Synonyms

bHLHb24;Class B basic helix-loop-helix protein 24;DNA-binding protein inhibitor ID-1;ID 1;ID1;Inhibitor of differentiation 1;Inhibitor of DNA binding 1

Gene names

ID1

Research area

Epigenetics and Nuclear Signaling

Target protein

ID1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5198Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.137 2.159 2.125
2.18
1200ng/L 1.884 1.813 1.778
1.741
600ng/L 1.145 1.192 1.158
1.238
300ng/L 0.664 0.639 0.605
0.614
150ng/L 0.384 0.398 0.364
0.412
0ng/L 0.041 0.034 0
0.028

Precision

We measured random samples of E5198Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 153 8.18 5.3
Intra-Assay 2 18 571.8 38.43 6.7
Intra-Assay 3 18 736.8 32.76 4.4

Protocols

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  • Size

    Code

    Price

  • 48T

    E5198Hu

    $320.00

  • 96T

    E5198Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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