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Home > Product > ELISA Kit > Human DNA-binding Protein Inhibitor Id-2, ID2 ELISA Kit

Human DNA-binding Protein Inhibitor Id-2, ID2 ELISA Kit

Human DNA-binding Protein Inhibitor Id-2, ID2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.48ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.48ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

DNA-Binding Protein Inhibitor ID-2

Code

E5199Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.48ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Restricts the CLOCK and ARNTL/BMAL1 localization to the cytoplasm. Plays a role in both the input and output pathways of the circadian clock: in the input component, is involved in modulating the magnitude of photic entrainment and in the output component, contributes to the regulation of a variety of liver clock-controlled genes involved in lipid metabolism.

UniProt accession

MASS(Da)

14,917

GeneID

3398

Synonyms

bHLHb26;Class B basic helix-loop-helix protein 26;DNA-binding protein inhibitor ID-2;ID 2;ID2;Inhibitor of differentiation 2;Inhibitor of DNA binding 2

Gene names

ID2

Research area

Epigenetics and Nuclear Signaling

Target protein

ID2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5199Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.351 2.402 2.328
2.452
800ng/L 1.233 1.259 1.186
1.284
400ng/L 0.722 0.746 0.673
0.77
200ng/L 0.443 0.489 0.416
0.536
100ng/L 0.25 0.287 0.214
0.323
0ng/L 0.078 0.073 0
0.069

Precision

We measured random samples of E5199Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1011.4 33.39 3.3
Intra-Assay 2 18 355.5 14.68 4.1
Intra-Assay 3 18 594.7 34.82 5.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E5199Hu

    $320.00

  • 96T

    E5199Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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