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Home > Product > ELISA Kit > Human Dual Specificity Mitogen-activated Protein Kinase 4, MAP2K4 ELISA Kit

Human Dual Specificity Mitogen-activated Protein Kinase 4, MAP2K4 ELISA Kit

Human Dual Specificity Mitogen-activated Protein Kinase 4, MAP2K4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 6.62ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 6.62ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Dual Specificity Mitogen-Activated Protein kinase 4

Code

E5205Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

6.62ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14.

UniProt accession

MASS(Da)

44,288

GeneID

6416

Synonyms

c-Jun N-terminal kinase 1;Dual specificity mitogen-activated protein kinase 4;JNK-activating kinase 1;JNKK;MAP kinase 4;MAP2K4;MAPK/ERK kinase 4;MAPKK 4;MEK 4;SAPK kinase 1;SAPK/ERK kinase 1;SAPKK1;SAPKK-1;SEK1;Stress-activated protein kinase 1

Gene names

MAP2K4

Research area

Signal Transduction

Target protein

MAP2K4

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5205Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 1.699 1.786 1.748
1.873
800ng/L 0.87 0.946 0.908
1.023
400ng/L 0.572 0.494 0.456
0.416
200ng/L 0.276 0.219 0.181
0.161
100ng/L 0.144 0.133 0.095
0.122
0ng/L 0.038 0.038 0
0.037

Precision

We measured random samples of E5205Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 938.1 42.71 4.6
Intra-Assay 2 18 1645.3 65.64 4
Intra-Assay 3 18 531.6 27.51 5.2

Protocols

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  • Size

    Code

    Price

  • 48T

    E5205Hu

    $320.00

  • 96T

    E5205Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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