E3 ubiquitin-protein ligase AMFR

E5210Hu

Sandwich

48T,96T

7.15ng/L

15-3000ng/L

Serum, plasma, cell culture supernates

Human

2-8ºC

1h 30m

E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins, such as CD3D, CYP3A4, CFTR, INSIG1, SOAT2/ACAT2 and APOB for proteasomal degradation (PubMed: 10456327, PubMed: 11724934, PubMed: 12670940, PubMed: 19103148, PubMed: 24424410, PubMed: 28604676). Component of a VCP/p97-AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD) (PubMed: 10456327, PubMed: 11724934, PubMed: 19103148, PubMed: 24424410). The VCP/p97-AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG1 complex at the ER membrane (PubMed: 16168377, PubMed: 22143767). In addition, interaction of AMFR with AUP1 facilitates interaction of AMFR with ubiquitin-conjugating enzyme UBE2G2 and ubiquitin ligase RNF139, leading to sterol-induced HMGCR ubiquitination (PubMed: 23223569). The ubiquitinated HMGCR is then released from the ER into the cytosol for subsequent destruction (PubMed: 16168377, PubMed: 22143767, PubMed: 23223569). In addition to ubiquitination on lysine residues, catalyzes ubiquitination on cysteine residues: together with INSIG1, mediates polyubiquitination of SOAT2/ACAT2 at 'Cys-277', leading to its degradation when the lipid levels are low (PubMed: 28604676). Catalyzes ubiquitination and subsequent degradation of INSIG1 when cells are depleted of sterols (PubMed: 17043353). Mediates polyubiquitination of INSIG2 at 'Cys-215' in some tissues, leading to its degradation (PubMed: 31953408). Also regulates ERAD through the ubiquitination of UBL4A a component of the BAG6/BAT3 complex (PubMed: 21636303). Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation (PubMed: 21636303). Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine motility factor (PubMed: 10456327). In association with LMBR1L and UBAC2, negatively regulates the canonical Wnt signaling pathway in the lymphocytes by promoting the ubiquitin-mediated degradation of CTNNB1 and Wnt receptors FZD6 and LRP6 (PubMed: 31073040).

72,996

267

AMF receptor;AMFR;Autocrine motility factor receptor;E3 ubiquitin-protein ligase AMFR;gp78;RING finger protein 45

AMFR

Cell Biology

AMFR