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Home > Product > ELISA Kit > Human E3 Ubiquitin-protein Ligase Amfr, AMFR ELISA Kit

Human E3 Ubiquitin-protein Ligase Amfr, AMFR ELISA Kit

Human E3 Ubiquitin-protein Ligase Amfr, AMFR ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.15ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.15ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

E3 ubiquitin-protein ligase AMFR

Code

E5210Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.15ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins, such as CD3D, CYP3A4, CFTR, INSIG1, SOAT2/ACAT2 and APOB for proteasomal degradation (PubMed: 10456327, PubMed: 11724934, PubMed: 12670940, PubMed: 19103148, PubMed: 24424410, PubMed: 28604676). Component of a VCP/p97-AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD) (PubMed: 10456327, PubMed: 11724934, PubMed: 19103148, PubMed: 24424410). The VCP/p97-AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG1 complex at the ER membrane (PubMed: 16168377, PubMed: 22143767). In addition, interaction of AMFR with AUP1 facilitates interaction of AMFR with ubiquitin-conjugating enzyme UBE2G2 and ubiquitin ligase RNF139, leading to sterol-induced HMGCR ubiquitination (PubMed: 23223569). The ubiquitinated HMGCR is then released from the ER into the cytosol for subsequent destruction (PubMed: 16168377, PubMed: 22143767, PubMed: 23223569). In addition to ubiquitination on lysine residues, catalyzes ubiquitination on cysteine residues: together with INSIG1, mediates polyubiquitination of SOAT2/ACAT2 at 'Cys-277', leading to its degradation when the lipid levels are low (PubMed: 28604676). Catalyzes ubiquitination and subsequent degradation of INSIG1 when cells are depleted of sterols (PubMed: 17043353). Mediates polyubiquitination of INSIG2 at 'Cys-215' in some tissues, leading to its degradation (PubMed: 31953408). Also regulates ERAD through the ubiquitination of UBL4A a component of the BAG6/BAT3 complex (PubMed: 21636303). Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation (PubMed: 21636303). Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine motility factor (PubMed: 10456327). In association with LMBR1L and UBAC2, negatively regulates the canonical Wnt signaling pathway in the lymphocytes by promoting the ubiquitin-mediated degradation of CTNNB1 and Wnt receptors FZD6 and LRP6 (PubMed: 31073040).

UniProt accession

MASS(Da)

72,996

GeneID

267

Synonyms

AMF receptor;AMFR;Autocrine motility factor receptor;E3 ubiquitin-protein ligase AMFR;gp78;RING finger protein 45

Gene names

AMFR

Research area

Cell Biology

Target protein

AMFR

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5210Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.106 2.23 2.198
2.354
800ng/L 1.493 1.539 1.506
1.584
400ng/L 1.187 1.165 1.133
1.143
200ng/L 0.644 0.645 0.613
0.647
100ng/L 0.411 0.449 0.417
0.488
0ng/L 0.034 0.032 0
0.03

Precision

We measured random samples of E5210Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E5210Hu

    $320.00

  • 96T

    E5210Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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