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Home > Product > ELISA Kit > Human Endoplasmic Reticulum Aminopeptidase 1, ERAP1 ELISA Kit

Human Endoplasmic Reticulum Aminopeptidase 1, ERAP1 ELISA Kit

Human Endoplasmic Reticulum Aminopeptidase 1, ERAP1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Endoplasmic Reticulum Aminopeptidase 1

Code

E5237Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.024ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Aminopeptidase that plays a central role in Peptide trimming, a step required for the generation of most HLA class I-binding Peptides. Peptide trimming is essential to customize longer precursor Peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and Peptides with a hydrophobic C-terminus, while it has weak activity toward Peptides with charged C-terminus. May play a role in the inactivation of Peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney.

UniProt accession

MASS(Da)

107,235

GeneID

51752

Synonyms

Adipocyte-derived leucine aminopeptidase;A-LAP;Aminopeptidase PILS;ARTS-1;Endoplasmic reticulum aminopeptidase 1;ERAP 1;ERAP1;PILS-AP;Puromycin-insensitive leucyl-specific aminopeptidase;Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator

Gene names

ERAP1

Research area

Immunology

Target protein

ERAP1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5237Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.599 2.505 2.454
2.41
6ng/ml 1.435 1.466 1.416
1.497
3ng/ml 0.791 0.841 0.791
0.892
1.5ng/ml 0.415 0.435 0.385
0.456
0.75ng/ml 0.228 0.241 0.191
0.255
0ng/ml 0.045 0.05 0
0.056

Precision

We measured random samples of E5237Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6.8 0.19 2.8
Intra-Assay 2 18 1.2 0.06 5.1
Intra-Assay 3 18 3 0.18 6

Protocols

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Associated products

  • Size

    Code

    Price

  • 48T

    E5237Hu

    $320.00

  • 96T

    E5237Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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