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Home > Product > ELISA Kit > Canine Interleukin 33, IL-33 ELISA Kit

Canine Interleukin 33, IL-33 ELISA Kit

Canine Interleukin 33, IL-33 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 6.39ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Canine

Product Summary

Size: 96T,48T

Sensitivity: 6.39ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Canine

Product overview

Full product name

Interleukin 33

Code

E0353Ca

Assay type

Sandwich

Size

96T,48T

Sensitivity

6.39ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Canine

Storage

2-8ºC

Assay time

1h 30m

Background

Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury (By similarity). In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation (By similarity).

UniProt accession

MASS(Da)

30,179

GeneID

403810

Synonyms

IL 33;IL33;IL-33;Interleukin-33;Protein DVS27

Gene names

IL33

Research area

Cardiovascular; Immunology

Target protein

IL33

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0353Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.129 2.24 2.198
2.35
800ng/L 1.577 1.641 1.599
1.705
400ng/L 1.114 1.118 1.077
1.123
200ng/L 0.677 0.686 0.644
0.694
100ng/L 0.452 0.472 0.43
0.491
0ng/L 0.056 0.042 0
0.027

Precision

We measured random samples of E0353Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 795.8 45.15 5.7
Intra-Assay 2 18 107.5 7.24 6.7
Intra-Assay 3 18 264.3 18.45 7

Protocols

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  • Size

    Code

    Price

  • 96T

    E0353Ca

    $475.00

  • 48T

    E0353Ca

    $350.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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