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Home > Product > ELISA Kit > Human Ephrin-B2, EFNB2 ELISA Kit

Human Ephrin-B2, EFNB2 ELISA Kit

Human Ephrin-B2, EFNB2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.29ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 5.29ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Ephrin-B2

Code

E5254Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.29ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons. (Microbial infection) Acts as a receptor for Hendra virus and Nipah virus.

UniProt accession

MASS(Da)

36,923

GeneID

1948

Synonyms

EFNB 2;EFNB2;EPH-related receptor tyrosine kinase ligand 5;Ephrin-B2;HTK ligand;HTK-L;LERK-5

Gene names

EFNB2

Research area

Cardiovascular

Target protein

EFNB2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5254Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1280ng/L 2.285 2.324 2.273
2.363
640ng/L 1.165 1.197 1.146
1.228
320ng/L 0.706 0.669 0.618
0.631
160ng/L 0.465 0.47 0.419
0.475
80ng/L 0.259 0.231 0.18
0.203
0ng/L 0.061 0.051 0
0.04

Precision

We measured random samples of E5254Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 574 21.66 3.8
Intra-Assay 2 18 750.3 21.44 2.9
Intra-Assay 3 18 253.1 15.78 6.2

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E5254Hu

    $320.00

  • 96T

    E5254Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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