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Home > Product > ELISA Kit > Human Eukaryotic Translation Initiation Factor 4B, EIF4B ELISA Kit

Human Eukaryotic Translation Initiation Factor 4B, EIF4B ELISA Kit

Human Eukaryotic Translation Initiation Factor 4B, EIF4B ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.81ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.81ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Eukaryotic Translation Initiation Factor 4B

Code

E5267Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.81ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

UniProt accession

MASS(Da)

69,151

GeneID

1975

Synonyms

EIF 4B;EIF4B;eIF-4B;Eukaryotic translation initiation factor 4B

Gene names

EIF4B

Research area

Epigenetics and Nuclear Signaling

Target protein

EIF4B

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5267Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.047 2.155 2.111
2.262
800ng/L 1.144 1.223 1.18
1.302
400ng/L 0.633 0.703 0.659
0.772
200ng/L 0.519 0.461 0.417
0.403
100ng/L 0.188 0.207 0.164
0.226
0ng/L 0.042 0.043 0
0.045

Precision

We measured random samples of E5267Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 699 28.02 4
Intra-Assay 2 18 1327.2 36.56 2.8
Intra-Assay 3 18 244.4 13.83 5.7

Protocols

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Associated products

  • Size

    Code

    Price

  • 48T

    E5267Hu

    $320.00

  • 96T

    E5267Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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