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Home > Product > ELISA Kit > Human Eyes Absent Homolog 2, EYA2 ELISA Kit

Human Eyes Absent Homolog 2, EYA2 ELISA Kit

Human Eyes Absent Homolog 2, EYA2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.25ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 2.25ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Eyes Absent Homolog 2

Code

E5272Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.25ng/L

Detection range

5-1000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5 (PubMed: 12500905, PubMed: 23435380). Tyrosine phosphatase that dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress (PubMed: 19351884). Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis. Plays an important role in hypaxial muscle development together with SIX1 and DACH2; in this it is functionally redundant with EYA1 (PubMed: 12500905).

UniProt accession

MASS(Da)

59,232

GeneID

2139

Synonyms

EYA 2;EYA2;Eyes absent homolog 2

Gene names

EYA2

Research area

Signal Transduction

Target protein

EYA2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5272Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
640ng/L 2.166 2.101 2.067
2.036
320ng/L 1.45 1.412 1.377
1.373
160ng/L 0.549 0.562 0.527
0.574
80ng/L 0.39 0.362 0.327
0.334
40ng/L 0.213 0.187 0.153
0.161
0ng/L 0.023 0.034 0
0.046

Precision

We measured random samples of E5272Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 113.2 5.06 4.5
Intra-Assay 2 18 456.8 20.55 4.5
Intra-Assay 3 18 215.2 8.5 4

Protocols

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Associated products

  • Size

    Code

    Price

  • 48T

    E5272Hu

    $320.00

  • 96T

    E5272Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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