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Home > Product > ELISA Kit > Canine Metalloproteinase Inhibitor 1, TIMP1 ELISA Kit

Canine Metalloproteinase Inhibitor 1, TIMP1 ELISA Kit

Canine Metalloproteinase Inhibitor 1, TIMP1 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 9.59ng/mL

Detection range: 20-4500ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Canine

Product Summary

Size: 96T,48T

Sensitivity: 9.59ng/mL

Detection range: 20-4500ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Canine

Product overview

Full product name

Metalloproteinase Inhibitor 1

Code

E0358Ca

Assay type

Sandwich

Size

96T,48T

Sensitivity

9.59ng/mL

Detection range

20-4500ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Canine

Storage

2-8ºC

Assay time

1h 30m

Background

Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling.

UniProt accession

MASS(Da)

22,852

GeneID

403816

Synonyms

Metalloproteinase inhibitor 1;TIMP 1;TIMP1;TIMP-1;Tissue inhibitor of metalloproteinases 1

Gene names

TIMP1

Research area

Others

Target protein

TIMP1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0358Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/mL 1.788 1.868 1.824
1.948
1200ng/mL 1.04 1.034 0.99
1.028
600ng/mL 0.621 0.619 0.575
0.617
300ng/mL 0.189 0.214 0.17
0.239
150ng/mL 0.08 0.076 0.033
0.073
0ng/mL 0.036 0.044 0
0.052

Precision

We measured random samples of E0358Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 512.1 23.94 4.7
Intra-Assay 2 18 1252.7 49.73 4
Intra-Assay 3 18 2217.7 75.91 3.4

Protocols

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  • Size

    Code

    Price

  • 96T

    E0358Ca

    $475.00

  • 48T

    E0358Ca

    $350.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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