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Home > Product > ELISA Kit > Human G Protein-coupled Bile Acid Receptor 1, GPBAR1 ELISA Kit

Human G Protein-coupled Bile Acid Receptor 1, GPBAR1 ELISA Kit

Human G Protein-coupled Bile Acid Receptor 1, GPBAR1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.027ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.027ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

G Protein-Coupled Bile Acid Receptor 1

Code

E5310Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.027ng/mL

Detection range

0.05-20ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Receptor for bile acid. Bile acid-binding induces its internalization, activation of extracellular signal-regulated kinase and intracellular cAMP production. May be involved in the suppression of macrophage functions by bile acids.

UniProt accession

MASS(Da)

35,248

GeneID

151306

Synonyms

BG37;GPBAR 1;GPBAR1;G-protein coupled bile acid receptor 1;G-protein coupled receptor GPCR19;hBG37;hGPCR19;M-BAR;Membrane-type receptor for bile acids;

Gene names

GPBAR1

Research area

Signal Transduction

Target protein

GPBAR1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5310Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12.8ng/mL 2.05 2.092 2.041
2.134
6.4ng/mL 1.026 1.027 0.976
1.028
3.2ng/mL 0.602 0.519 0.468
0.436
1.6ng/mL 0.198 0.254 0.203
0.31
0.8ng/mL 0.118 0.112 0.061
0.106
0ng/mL 0.052 0.051 0
0.05

Precision

We measured random samples of E5310Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 4.7 0.17 3.6
Intra-Assay 2 18 8.5 0.23 2.7
Intra-Assay 3 18 4 0.23 5.8

Protocols

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  • Size

    Code

    Price

  • 48T

    E5310Hu

    $320.00

  • 96T

    E5310Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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