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Home > Product > ELISA Kit > Human G Protein-coupled Receptor Kinase 5, GRK5 ELISA Kit

Human G Protein-coupled Receptor Kinase 5, GRK5 ELISA Kit

Human G Protein-coupled Receptor Kinase 5, GRK5 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 10.14ng/L

Detection range: 20-3800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 10.14ng/L

Detection range: 20-3800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

G Protein-Coupled Receptor Kinase 5

Code

E5311Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

10.14ng/L

Detection range

20-3800ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro).

UniProt accession

MASS(Da)

67,787

GeneID

2869

Synonyms

G protein-coupled receptor kinase 5;G protein-coupled receptor kinase GRK5;GRK 5;GRK5

Gene names

GRK5

Research area

Signal Transduction

Target protein

GRK5

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5311Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 2 2.071 2.043
2.142
1000ng/L 1.568 1.476 1.448
1.385
500ng/L 0.58 0.608 0.579
0.635
250ng/L 0.441 0.391 0.362
0.341
125ng/L 0.13 0.172 0.143
0.214
0ng/L 0.022 0.029 0
0.036

Precision

We measured random samples of E5311Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 383.9 16.73 4.4
Intra-Assay 2 18 972 33.57 3.5
Intra-Assay 3 18 651.9 26.45 4.1

Protocols

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  • Size

    Code

    Price

  • 48T

    E5311Hu

    $320.00

  • 96T

    E5311Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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