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Home > Product > ELISA Kit > Human Gamma-secretase Subunit Aph-1A, APH1A ELISA Kit

Human Gamma-secretase Subunit Aph-1A, APH1A ELISA Kit

Human Gamma-secretase Subunit Aph-1A, APH1A ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.32ng/L

Detection range: 20-3800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.32ng/L

Detection range: 20-3800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Gamma-Secretase Subunit Aph-1A

Code

E5319Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.32ng/L

Detection range

20-3800ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Non-catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) (PubMed: 12297508, PubMed: 12522139, PubMed: 12763021, PubMed: 12679784, PubMed: 25043039, PubMed: 26280335, PubMed: 30598546, PubMed: 30630874). Required for normal gamma-secretase assembly (PubMed: 12522139, PubMed: 12471034, PubMed: 12763021, PubMed: 19369254). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels (Probable).

UniProt accession

MASS(Da)

28,996

GeneID

51107

Synonyms

APH1A;APH-1a;Aph-1alpha;Gamma-secretase subunit APH-1A;Presenilin-stabilization factor

Gene names

APH1A

Research area

Others

Target protein

APH1A

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5319Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 1.979 1.827 1.8
1.674
1000ng/L 0.895 0.962 0.935
1.03
500ng/L 0.499 0.535 0.508
0.572
250ng/L 0.141 0.166 0.139
0.19
125ng/L 0.068 0.063 0.036
0.058
0ng/L 0.03 0.027 0
0.024

Precision

We measured random samples of E5319Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 765.8 37.86 4.9
Intra-Assay 2 18 1277.5 35.8 2.8
Intra-Assay 3 18 378.8 17 4.5

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E5319Hu

    $320.00

  • 96T

    E5319Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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