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Home > Product > ELISA Kit > Human Glutaredoxin-2, Mitochondrial, GLRX2 ELISA Kit

Human Glutaredoxin-2, Mitochondrial, GLRX2 ELISA Kit

Human Glutaredoxin-2, Mitochondrial, GLRX2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.52ng/L

Detection range: 1-400ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.52ng/L

Detection range: 1-400ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Glutaredoxin-2, Mitochondrial

Code

E5342Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.52ng/L

Detection range

1-400ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Glutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress. Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release.

UniProt accession

MASS(Da)

18,052

GeneID

51022

Synonyms

GLRX 2;GLRX2;Glutaredoxin-2, mitochondrial

Gene names

GLRX2

Research area

Others

Target protein

GLRX2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5342Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
240ng/L 2.019 1.865 1.83
1.711
120ng/L 0.968 0.954 0.919
0.941
60ng/L 0.615 0.585 0.55
0.554
30ng/L 0.207 0.229 0.194
0.252
15ng/L 0.139 0.139 0.104
0.139
0ng/L 0.024 0.035 0
0.046

Precision

We measured random samples of E5342Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 225.7 6.25 2.8
Intra-Assay 2 18 133.2 6.37 4.8
Intra-Assay 3 18 85.3 5.23 6.1

Protocols

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  • Size

    Code

    Price

  • 48T

    E5342Hu

    $320.00

  • 96T

    E5342Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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