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Home > Product > ELISA Kit > Canine Tyrosinase, TYR ELISA Kit

Canine Tyrosinase, TYR ELISA Kit

Canine Tyrosinase, TYR ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 9.32ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Canine

Product Summary

Size: 96T,48T

Sensitivity: 9.32ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Canine

Product overview

Full product name

Tyrosinase

Code

E0375Ca

Assay type

Sandwich

Size

96T,48T

Sensitivity

9.32ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Canine

Storage

2-8ºC

Assay time

1h 30m

Background

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds (By similarity). Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine (By similarity). In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone (By similarity).

UniProt accession

MASS(Da)

60,336

GeneID

403405

Synonyms

Monophenol monooxygenase;TYR;Tyrosinase

Gene names

TYR

Research area

Others

Target protein

TYR

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0375Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.21 2.265 2.225
2.319
1200ng/L 1.321 1.252 1.211
1.182
600ng/L 0.807 0.797 0.757
0.788
300ng/L 0.455 0.466 0.425
0.476
150ng/L 0.232 0.215 0.175
0.198
0ng/L 0.029 0.04 0
0.052

Precision

We measured random samples of E0375Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 585.2 43.59 7.4
Intra-Assay 2 18 1142.4 59.69 5.2
Intra-Assay 3 18 1764.5 57.83 3.3

Protocols

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  • Size

    Code

    Price

  • 96T

    E0375Ca

    $475.00

  • 48T

    E0375Ca

    $350.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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