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Home > Product > ELISA Kit > Human Histone Deacetylase 4, HDAC4 ELISA Kit

Human Histone Deacetylase 4, HDAC4 ELISA Kit

Human Histone Deacetylase 4, HDAC4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.48ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.48ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Histone Deacetylase 4

Code

E5410Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.48ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Deacetylates HSPA1A and HSPA1B at 'Lys-77' leading to their preferential binding to co-chaperone STUB1 (PubMed: 27708256).

UniProt accession

MASS(Da)

119,040

GeneID

9759

Synonyms

HD4;HDAC 4;HDAC4;Histone deacetylase 4

Gene names

HDAC4

Research area

Others

Target protein

HDAC4

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5410Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.155 2.156 2.11
2.157
1200ng/L 1.558 1.629 1.583
1.7
600ng/L 1.175 1.158 1.113
1.142
300ng/L 0.725 0.732 0.686
0.738
150ng/L 0.453 0.464 0.418
0.476
0ng/L 0.046 0.046 0
0.045

Precision

We measured random samples of E5410Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 700.2 51.32 7.3
Intra-Assay 2 18 1456.7 83.83 5.8
Intra-Assay 3 18 146.3 9.56 6.5

Protocols

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  • Size

    Code

    Price

  • 48T

    E5410Hu

    $320.00

  • 96T

    E5410Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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