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Home > Product > ELISA Kit > Human Histone Deacetylase 9, HDAC9 ELISA Kit

Human Histone Deacetylase 9, HDAC9 ELISA Kit

Human Histone Deacetylase 9, HDAC9 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.044ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.044ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Histone Deacetylase 9

Code

E5411Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.044ng/mL

Detection range

0.05-20ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription. Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.

UniProt accession

MASS(Da)

111,297

GeneID

9734

Synonyms

HD7;HD7b;HD9;HDAC 9;HDAC9;Histone deacetylase 7B;Histone deacetylase 9;Histone deacetylase-related protein;MEF2-interacting transcription repressor MITR;

Gene names

HDAC9

Research area

Epigenetics and Nuclear Signaling

Target protein

HDAC9

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5411Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12.8ng/mL 2.165 2.144 2.114
2.122
6.4ng/mL 1.299 1.303 1.273
1.307
3.2ng/mL 0.748 0.778 0.748
0.807
1.6ng/mL 0.527 0.485 0.456
0.444
0.8ng/mL 0.183 0.196 0.167
0.209
0ng/mL 0.028 0.03 0
0.031

Precision

We measured random samples of E5411Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 7.8 0.34 4.3
Intra-Assay 2 18 3.9 0.27 6.8
Intra-Assay 3 18 1.8 0.1 5.3

Protocols

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  • Size

    Code

    Price

  • 48T

    E5411Hu

    $320.00

  • 96T

    E5411Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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