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Home > Product > ELISA Kit > Human Hyaluronidase Ph-20, SPAM1 ELISA Kit

Human Hyaluronidase Ph-20, SPAM1 ELISA Kit

Human Hyaluronidase Ph-20, SPAM1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 4.95ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 4.95ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Hyaluronidase Ph-20

Code

E5438Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

4.95ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

UniProt accession

MASS(Da)

57,848

GeneID

6677

Synonyms

Hyal-PH20;Hyaluronidase PH-20;Hyaluronoglucosaminidase PH-20;SPAM 1;SPAM1;Sperm adhesion molecule 1;Sperm surface protein PH-20

Gene names

SPAM1

Research area

Signal Transduction

Target protein

SPAM1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5438Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.018 1.936 1.898
1.854
600ng/L 1.095 1.073 1.034
1.05
300ng/L 0.419 0.517 0.479
0.616
150ng/L 0.175 0.192 0.153
0.209
75ng/L 0.122 0.135 0.096
0.147
0ng/L 0.052 0.039 0
0.025

Precision

We measured random samples of E5438Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 255 12.29 4.8
Intra-Assay 2 18 839.6 25.87 3.1
Intra-Assay 3 18 508 19.35 3.8

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E5438Hu

    $320.00

  • 96T

    E5438Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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