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Home > Product > ELISA Kit > Human Interleukin Enhancer-binding Factor 3, ILF3 ELISA Kit

Human Interleukin Enhancer-binding Factor 3, ILF3 ELISA Kit

Human Interleukin Enhancer-binding Factor 3, ILF3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 4.84ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 4.84ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Interleukin Enhancer-Binding Factor 3

Code

E5503Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

4.84ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

RNA-binding protein that plays an essential role in the biogenesis of circular RNAs (circRNAs) which are produced by back-splicing circularization of pre-mRNAs. Within the nucleus, promotes circRNAs processing by stabilizing the regulatory elements residing in the flanking introns of the circularized exons. Plays thereby a role in the back-splicing of a subset of circRNAs (PubMed: 28625552). As a consequence, participates in a wide range of transcriptional and post-transcriptional processes. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed: 14731398). Upon viral infection, ILF3 accumulates in the cytoplasm and participates in the innate antiviral response (PubMed: 21123651). Mechanistically, ILF3 becomes phosphorylated and activated by the double-stranded RNA-activated protein kinase/PKR which releases ILF3 from cellular mature circRNAs. In turn, unbound ILF3 molecules are able to interact with and thus inhibit viral mRNAs (PubMed: 21123651, PubMed: 28625552).

UniProt accession

MASS(Da)

95,338

GeneID

3609

Synonyms

Double-stranded RNA-binding protein 76;DRBP76;ILF 3;ILF3;Interleukin enhancer-binding factor 3;M-phase phosphoprotein 4;MPP4;NFAR;NF-AT-90;Nuclear factor associated with dsRNA;Nuclear factor of activated T-cells 90 kDa;TCP80;Translational control protein 80;

Gene names

ILF3

Research area

Signal Transduction

Target protein

ILF3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5503Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.076 2.126 2.083
2.175
600ng/L 1.925 1.83 1.787
1.736
300ng/L 1.092 1.122 1.079
1.152
150ng/L 0.747 0.744 0.701
0.741
75ng/L 0.375 0.408 0.365
0.441
0ng/L 0.03 0.043 0
0.055

Precision

We measured random samples of E5503Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 291.8 17.2 5.9
Intra-Assay 2 18 519.1 29.15 5.6
Intra-Assay 3 18 134.6 9.75 7.2

Protocols

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  • Size

    Code

    Price

  • 48T

    E5503Hu

    $320.00

  • 96T

    E5503Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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